Protocol for clumped isotopes - weighing and running measurements

Introduction

The following protocol describes how to weigh samples and run clumped isotopes measurements.

Safety Information

Wear cryo-gloves, long sleeve shirts, protection glasses and closed shoes when handling liquid nitrogen in open dewars. Liquid nitrogen is extremely cold. The vapor of liquid nitrogen can rapidly freeze skin tissue and eye fluid, resulting in cold burns, frostbite, and permanent eye damage even by brief exposure.

In the event of spillage of liquid nitrogen on a person: If exposed to liquid or cold gas, restore tissue to normal body temperature, 37°C, followed by protection of the injured tissue from further damage and infection. Remove or loosen clothing that may constrict blood circulation to the frozen area. Rapid warming of the affected part is best achieved by using water at 42°C. Water should under no circumstances be over 44°C, nor should the frozen part be rubbed either before or after rewarming. If in doubt contact Legevakt (phone 116 117 or 113 for emergencies).

If someone gets liquid nitrogen it in their eyes, call 113, wash the eyes with appropriate equipment immediately.