Protocol for saltwater stable isotope analysis on Picarro: Difference between revisions
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===Liquid run setup salt water=== | |||
'''1. Prepare standards''' | '''1. Prepare standards''' | ||
- Use BERM for drift | - Use BERM for drift | ||
Revision as of 14:10, 1 September 2020
Liquid run setup salt water
1. Prepare standards
- Use BERM for drift
- DI2 and EVAP2 for sample calibration
- Prepare once in inset vials before a set of runs
2. Run setup
- Order DI2, BERM, EVAP2
- Blocks of 8 samples
- BERM drift standard
- Repeat 3x for total 24 samples, with 2 drift samples in between, takes up 29 spots
- Rinse syringe with extra sample in position #43, after 10 samples
3. Select method
- Single wet peak, requires 2 extra injections, peak goes to waste (salt)
- FARLAB_Rinse_2, strokes, small injection
4. Number of Injections
- Standards: 14 injections (12 in data file)
- Samples: 8 injections (6 in data file)
5. Rinsing method
- 5 injections, results in one small injection of rinse sample due to single wet peak routine
6. Equipment cleaning
- Salt mesh exchanged after 24 samples, clean in ultrasonic bath, dry in glass oven, keep with septa
- Septum washed after each run, exchanged if needed after inspection
- Syringe washing in DI before and after run, repeated strokes until flow is smooth, moving plunger up and down over whole volume