Protocol for saltwater stable isotope analysis on Picarro: Difference between revisions
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===Liquid run setup salt water=== | |||
'''1. Prepare standards''' | '''1. Prepare standards''' | ||
- Use BERM for drift<br> | |||
- Use DI2 and EVAP2 for sample calibration<br> | |||
- Prepare once for a whole set of runs, using inset vials (300 uL)<br> | |||
<br> | |||
'''2. Run setup''' | '''2. Run setup''' | ||
- | - Sequence from DI2 to BERM to EVAP2<br> | ||
- Define blocks of 8 samples<br> | |||
- | - Insert BERM as drift standard after 8 samples<br> | ||
- Repeat 3x for total 24 samples, with 2 drift samples in between, takes up 29 spots<br> | |||
- BERM drift standard | - Rinse syringe with extra sample in position #43, after 10 samples<br> | ||
<br> | |||
- Repeat 3x for total 24 samples, with 2 drift samples in between, takes up 29 spots | |||
- Rinse syringe with extra sample in position #43, after 10 samples | |||
'''3. Select method''' | '''3. Select method''' | ||
- Single wet peak, requires 2 extra injections, peak goes to waste (salt) | - Single wet peak, requires 2 extra injections, peak goes to waste (salt)<br> | ||
- FARLAB_Rinse_2, strokes, small injection<br> | |||
- FARLAB_Rinse_2, strokes, small injection | <br> | ||
'''4. Number of Injections''' | '''4. Number of Injections''' | ||
- Standards: 14 injections (12 in data file) | - Standards: 14 injections (12 in data file)<br> | ||
- Samples: 8 injections (6 in data file)<br> | |||
- Samples: 8 injections (6 in data file) | <br> | ||
'''5. Rinsing method''' | '''5. Rinsing method''' | ||
- 5 injections, results in one small injection of rinse sample due to single wet peak routine | - 5 injections, results in one small injection of rinse sample due to single wet peak routine<br> | ||
<br> | |||
'''6. Equipment cleaning''' | '''6. Equipment cleaning''' | ||
- Salt mesh exchanged after 24 samples, clean in ultrasonic bath, dry in glass oven, keep with septa | - Salt mesh exchanged after 24 samples, clean in ultrasonic bath, dry in glass oven, keep with septa<br> | ||
- Septum washed after each run, exchanged if needed after inspection<br> | |||
- Septum washed after each run, exchanged if needed after inspection | - Syringe washing in DI before and after run, repeated strokes until flow is smooth, moving plunger up and down over whole volume<br> | ||
- Syringe washing in DI before and after run, repeated strokes until flow is smooth, moving plunger up and down over whole volume | |||
Latest revision as of 14:14, 1 September 2020
Liquid run setup salt water
1. Prepare standards
- Use BERM for drift
- Use DI2 and EVAP2 for sample calibration
- Prepare once for a whole set of runs, using inset vials (300 uL)
2. Run setup
- Sequence from DI2 to BERM to EVAP2
- Define blocks of 8 samples
- Insert BERM as drift standard after 8 samples
- Repeat 3x for total 24 samples, with 2 drift samples in between, takes up 29 spots
- Rinse syringe with extra sample in position #43, after 10 samples
3. Select method
- Single wet peak, requires 2 extra injections, peak goes to waste (salt)
- FARLAB_Rinse_2, strokes, small injection
4. Number of Injections
- Standards: 14 injections (12 in data file)
- Samples: 8 injections (6 in data file)
5. Rinsing method
- 5 injections, results in one small injection of rinse sample due to single wet peak routine
6. Equipment cleaning
- Salt mesh exchanged after 24 samples, clean in ultrasonic bath, dry in glass oven, keep with septa
- Septum washed after each run, exchanged if needed after inspection
- Syringe washing in DI before and after run, repeated strokes until flow is smooth, moving plunger up and down over whole volume