View source for Protocol for saltwater stable isotope analysis on Picarro

===Liquid run setup salt water===

'''1. Prepare standards'''

- Use BERM for drift<br>
- Use DI2 and EVAP2 for sample calibration<br>
- Prepare once for a whole set of runs, using inset vials (300 uL)<br>
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'''2. Run setup'''

- Sequence from DI2 to BERM to EVAP2<br>
- Define blocks of 8 samples<br>
- Insert BERM as drift standard after 8 samples<br>
- Repeat 3x for total 24 samples, with 2 drift samples in between, takes up 29 spots<br>
- Rinse syringe with extra sample in position #43, after 10 samples<br>
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'''3. Select method'''

- Single wet peak, requires 2 extra injections, peak goes to waste (salt)<br>
- FARLAB_Rinse_2, strokes, small injection<br>
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'''4. Number of Injections'''

- Standards: 14 injections (12 in data file)<br>
- Samples: 8 injections (6 in data file)<br>
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'''5. Rinsing method'''

- 5 injections, results in one small injection of rinse sample due to single wet peak routine<br>
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'''6. Equipment cleaning'''

- Salt mesh exchanged after 24 samples, clean in ultrasonic bath, dry in glass oven, keep with septa<br>
- Septum washed after each run, exchanged if needed after inspection<br>
- Syringe washing in DI before and after run, repeated strokes until flow is smooth, moving plunger up and down over whole volume<br>